In Vitro Selection of Salinity Tolerance Callus of Dwarf Napier Grass (Pennisetum purpureum cv. Mott)

Abstract

Tissue culture for forage is a multiplication by improving the quality genetic of forage and adaptation to abiotic stress condition such as salinity stress. In this idea, different levels of salinity tolerance were generated. Multiple shoots as initial explants were isolated from aseptically shoot-tillers in the field, and cultured in vitro. Explants ware inducted into induction calli medium. Explants were transferred to Murashige Skoog (MS) medium, added with 5% coconut water and 2 mgL-1 2,4-D (dichlorophenoxyacetic acid). The planted explants were incubated for 21 days. Calli were induced in the proliferation medium with 50 μM CuSO4 and growth regulators i.e 0.1 mg L-1 2,4-D, and 2 mg L-1 BAP for 40 days. Regeneration medium with addition, and growth regulator 0.1 mg L-1 NAA and 2 mg L-1 BAP for 30 days. In the medium of proliferation and regeneration were added different salinity (NaCl) level. The treatments were 42.5 mM NaCl, 85 mM NaCl, 170 mM NaCl). The percentage of green-spot production, root formation, shoot, complete plantlet, morphologically of calli were recorded. The results indicated that morphologically and physiologically of calli added NaCl concentration (salinity) influenced growth on each treatment of dwarf napier grass (Pennisetum purpureum cv.Mott)