Abstract
In vitro Studies of wild yam also known as Dioscorea oppositifolia L. is reported. Direct organogenesis and indirect organogenesis of D. oppositifolia is achieved in this study. Shoot and root were produced from nodal explants with Murashige and Skoog (MS) medium supplemented with 0.5mg/l 6benzylaminopurine (BAP) and 0.1mg/l NAA. Callus was successfully produced from leaf explants on Murashige and Skoog (MS) medium supplemented with 0.5mg/l indole-3-butyric acid (IBA).Multiple shoots were initiated from callus in Murashige and Skoog (MS) medium supplemented with-2.0mg/l Naphthalene acetic acid (NAA) and 2.5mg/i kinetin. Root induction was also achieved simultaneously from the base of the shoots in the same medium. Regenerants acclimatized in soil-rite showed vigorous shoot growth (within 2 weeks) and after 5 6 months were suitable for planting.
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