In vitro research on the biocompatibility of endothelial progenitor cells combined with bladder a-cellular matrix

Abstract

Objective By using endothelial progenitor cells combined with bladder acellalar ma-trix to construct a tissue engineered bladder. Methods Porcine peripheral endothelial progenitor cells were isolated and cultured, and the bladder acellular matrix was prepared. The biocompatibility of endothe-lial progenitor cells and bladder acellular matrix was detected in vitro. The endothelial progenitor cells co-combined with the bladder acellular matrix were observed in vitro. Results Endothelial progenitor cells were isolated and cultured successfully, and the cell markers included CD133 (25.1%), CD34 (55.9%), flk-1 (97.7%), CD31(82.0% ), CD144(95.4% ). The bladder acellular matrix was pre-pared. It was proven that the bladder acellular matrix had no significant cytotoxicity, and the cell vitality was more than 90%. Endothelial progenitor cells could proliferate and grow on the bladder acellular matrix in vitro. Conclusion Endothelial progenitor cells can serve as seeded cells, and bladder acellular matrix as a scaffold to construct tissue engineered bladder,which may offer a possible new approach to increase the vascularization in the tissue engineered bladder. Key words: Endothelial progenitor cells; Tissue engineering; Bladder; Biocompatibility; Vascularization