Abstract
Objective To investigate the feasibility of endothelial progenitor cells (EPCs) promoting angiogenesis of tissue-engineered sheet graft using bladder epithelial cells and smooth muscle cells seeded on the bladder acellular matrix (BAM) in vivo.Methods BAM of rabbits was made in vitro.Bladder epithelial cells,smooth muscle cells and EPCs of rabbits were cultured and expanded in vitro,then seeded on the BAM and cultured for 7 days for producing sheet graft.The experimental group was bladder epithelial cells-smooth muscle cells-EPCs-BAM sheet graft and the control group was bladder epithelial cellssmooth muscle cells-BAM sheet graft.Then the grafts were implanted into the back of rabbits.Specimens were obtained at 8th week postoperatively and were examined histologically.Results Bladder epithelial cells,smooth muscle cells and EPCs were cultured and expanded in vitro.At 8th week after operation,histological detection of specimens showed angiogenesis and epithelial coverage in the experimental group.The epithelial cells gradually integrated with the BAM and smooth muscle cells grew well.In the control group,the graft showed contracture and discontinuous epithelial lay,and there was no obvious vascularization.Conclusion Tissue-engineered sheet graft constructed by EPCs,bladder epithelial cells and smooth muscle cells grows well in vivo.EPCs can promote angiogenesis of tissue-engineered sheet graft. Key words: Bladder acellular matrix; Epithelial cells; Endothelial progenitor ceils; Tissue engineering
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
