Abstract
Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. However, the maintenance of this species is currently challenging because of climatic changes in the plant’s habitat, intensive agricultural purposes, grazing, and the like. Gynogenesis is a promising method for micropropagation and a useful technic for genetic engineering. The objective of this study was to analyze the gynogenesis of V. turcica. The experiments described here were implemented during the flowering time of V. turcica (spring) using unpollinated ovaries. Modified MS and B5 induction media and different plant growth regulators (BAP, GA3, 2,4-D, and KIN) were compared. The comparison of both media (MS and B5) showed that MS medium supplemented with 2,4-D and KIN was the best medium for in vitro unfertilized ovary culture and gave the best result of regenerated plants per 30 ovaries, and 92% callus was obtained. Only the control groups showed very low scores or no plants. From plant regeneration and subcultures, a total of 60 plants were produced, all green. These results are of interest to the in vitro conservation of endangered plant species like V. turcica.
Highlights
Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress
V. turcica was grown from unfertilized ovary explants, which were taken from Nezahat Gökyiğit Botanical Garden (NGBG) of Istanbul in Turkey
Callus formation was observed from ovary samples of V. turcica in both media (MS and B5) (Table 2)
Summary
Vuralia turcica is an endemic plant species with a polycarpellary gynoecium and has the ability to survive in salt stress. Vuralia turcica (Tan et al 1983), Uysal et al (2014) is a rhizomatous plant species endemic to the region of Eber and Aksehir lakes in Turkey, belonging to the Fabaceae family, locally named as “Piyan.” It has the significant property of usually having 3 (2 or 4 being possible) ovaries within a flower bud, which makes the V. turcica unique in the Fabaceae family (Vural 2009) (Figure 1) It only has two main subpopulations, which are found at protected properties and is under the danger of extinction due to the conversion of its habitat into agricultural fields, the intervention of nearby wetlands, and the change of suitable microclimate (Vural 2009). The effects of culture conditions on cultured unfertilized ovaries of V. turcica were investigated
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