Abstract
Orthosiphon stamineus or known as Misai kucing is a popular herbal tea plant that helps in treating the ailments of kidney and bladder, diabetes mellitus and gout. Due to high demand of this medicinal plant, a large propagation of this plant is recommended. In this study, a protocol for regeneration of Orthosiphon stamineus using axillary bud as explant was established. Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) were evaluated for their effects on shoot induction. Results obtained revealed that the best shooting ability was observed when explants were cultured on MS medium supplemented with 0.2 ppm of BAP. Subsequently, shoot elongation of established shoot was obtained in medium containing gibberelic acid (GA3) with concentration of 0.5 ppm and 1.0 ppm. The best shoot elongation was achieved with medium supplemented with 0.5 ppm of GA3. Subsequently, the elongated explants were transformed to root induction medium with the addition of either 0.2 ppm of 1-naphthaleneacetic acid (NAA) or 0.5 ppm NAA. The explants showed positive response in medium supplemented with 0.2 ppm of NAA. From this study it could be confirmed that suitable concentration of plant growth regulators could be used in the regeneration of Orthosiphon stamineus through tissue culture technique.
Highlights
O. stamineus or Misai Kucing which comes from the Lamiaceae family is one of the medicinal herbs used in Southeast Asia [1]
In Myanmar, leaves of O. stamineus have been used as anti-diabetic drugs and remedy to treat urinary tract and renal disease, while in Japan, it has been consumed as java tea that helps to facilitate the detoxification inside the body [6]
Axillary buds were cultured on five concentrations of BAP (0 ppm, 0.2 ppm, 0.4 ppm, 0.6 ppm, 0.8 ppm and 1.0 ppm) to induce the formation of shoots
Summary
O. stamineus or Misai Kucing which comes from the Lamiaceae family is one of the medicinal herbs used in Southeast Asia [1] It is locally known as Misai Kucing because the features of its bluish flowers look like a cat’s whiskers. This herb was cultivated a long time ago and is grown as garden plant and for wild type plant it can be seen growing along the forest edges, roadsides and wastelands. O. stamineus plant contains various bio-active compounds that make this plant usable as herbal medicine Such properties are 5-hydroxy-6, 7, 3, 4-tetramethoxyflavone, 4, 5, 6, 7-tetramethoxyflavone, terpenoids, inositol, myo-inositol, carotenoids and saponins [4]. In Myanmar, leaves of O. stamineus have been used as anti-diabetic drugs and remedy to treat urinary tract and renal disease, while in Japan, it has been consumed as java tea that helps to facilitate the detoxification inside the body [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
