Abstract
Abstract P. grandiflora is a known ornamental plant with abundant flowering. The flowers exhibit varied coloration with distinct forms and simple folded petals and/or multiple. The objective of this work was to induce regeneration via organogenesis and in vitro flowering of P. grandiflora. Nodal segments of seedlings germinated in vitro were used as explant source for regeneration. Kinetin (KIN) and 6-Benzylaminopurine (BA) were used for the induction of organogenesis. The treatments supplemented with 1.0 and 1.5 mg L−1 BA induced the highest number of adventitious shoots with an average number of 7.0 (±1.55) e 5.4 (±0.83), respectively. The microcuttings obtained from regenerated shoots produced floral buds. The floral buds were located in the axillary and terminal regions of the microcuttings and developed in approximately 10 days of cultivation until the anthesis. The highest number of flower buds was observed in the presence of 0.75 mg L−1 of gibberellic acid. This study opens new perspectives for the establishment of biotechnological tools to be applied for this important ornamental species.
Highlights
Portulaca grandiflora Hook. commonly known as “ten o’clock”, is one of the most widely cultivated annual flower plants in the tropical world by virtue of its easy cultivation, abundant flowering and flowers of varied colors such as red, yellow, pink, purple, white, orange and/or mixed
The main regeneration pathway of P. grandiflora is organogenesis, which is induced mainly from nodal explants and stem apices grown in the presence of cytokinin (Srivastava and Joshi, 2009; Jain and Bashir, 2010)
The present study aimed to evaluate the performance of nodal explants of P. grandiflora and to establish an in vitro regeneration system via organogenesis as well as induce flowering in vitro from a culture of microcuttings from previously regenerated plants
Summary
Portulaca grandiflora Hook. commonly known as “ten o’clock”, is one of the most widely cultivated annual flower plants in the tropical world by virtue of its easy cultivation, abundant flowering and flowers of varied colors such as red, yellow, pink, purple, white, orange and/or mixed. Commonly known as “ten o’clock”, is one of the most widely cultivated annual flower plants in the tropical world by virtue of its easy cultivation, abundant flowering and flowers of varied colors such as red, yellow, pink, purple, white, orange and/or mixed. It is found in distinct forms, with simple, folded, or multiple petals (Jain and Bashir, 2010). This ornamental plant can be grown in small spaces, gardens and pots. The main regeneration pathway of P. grandiflora is organogenesis, which is induced mainly from nodal explants and stem apices grown in the presence of cytokinin (Srivastava and Joshi, 2009; Jain and Bashir, 2010)
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