Abstract
BackgroundSecuridaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. This plant is endangered because of high seed dormancy, low germination rate, and over exploitation. Therefore, micropropagation method is important to address these problems. The objective of this study is to develop a micropropagation protocol for S. longipedunculata from shoot tip explants. ResultsAmong different Clorox concentrations, seeds sterilized with 10% Clorox for 10 min resulted in 85% decontamination and 80% germination. Among different media used to evaluate the rate of seed germination, seeds that were de-coated and transversally cut at the tip and cultured on basal MS medium resulted in 100% germination. The highest percentage of shoot initiation (87%) was obtained on MS medium containing 1.0 mg/l 6-Benzylaminopurine (BAP). The highest mean shoot number per explant (8.5 ± 0.69) was achieved on MS multiplication medium containing 1.5 mg/l BAP in combination with 0.1 mg/l Indole-3-butyric acid (IBA). The highest mean number of roots per explant (3.73 ± 0.69) was obtained on MS medium containing 2.0 mg/l Indole-3-acetic-acid (IAA). Among plantlets transferred to greenhouse, 60% survived after acclimatization. ConclusionsThis micropropagation protocol can be used for mass propagation of S. longipedunculata that contributes to its conservation and genetic improvement.
Highlights
Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine
Effect of Clorox concentrations on seed germination rate The highest percentage of decontaminated seeds (90%) was obtained at 15% Clorox, but the germination percentage was reduced to 20% (Table 1)
Among those Clorox concentrations, 10% Clorox concentration resulted in 85% decontaminated seeds and 80% seed germination
Summary
Securidaca longipedunculata Fresen is an indigenous medicinal plant in Africa that has an important place in both traditional and modern medicine. It grows up to 12 m tall, with an often flattened or slightly fluted bole It is an indigenous medicinal plant in Africa and plays an important role in both traditional and modern medicine. This tree is commonly used as a pesticide against beetles in stored grains [1]. It is distributed in a wide range of climates ranging from subtropical, hot, and arid climate to summer rainfall and equatorial humid.
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