In Vitro Propagation of Pomegranate (Punica granatum L.)

Abstract

This study was conducted in the plant cell and tissue culture laboratory of the Department of Horticulture and Landscaping / College of Agriculture, University of Kirkuk – Iraq. To study in vitro propagation of pomegranate by adding (0.0, 0.5, 1.0, 1.5, and 2.0) mg .L−16-benzyladenine (BA) and (0.0, 2.0, 4.0, 6.0, and 8.0) mg .L−1Kinetin (Kin) on Initiation state. The results showed that the highest rate number of branches is 1.20 branches. Vegetative part −1 for the medium supplemented with 0.5 mg .L−1 BA, the highest average for the longest length branch was 2.00 cm, the highest average for the number of leaves was 6.40. Vegetative part −1 when cultivated in the medium supplemented with 1.5 mg.L−1 BA. Whereas, when treated with Kin, the highest rate of the number of branches was recorded, 0.70 branches, vegetative part−1. The length of the most extended branch was 1.00 cm, at a concentration of 4 mg .L−1. The highest average number of leaves was 3.40 leaves, vegetative part-1, when cultured with the medium supplied with 8 mg. L −1.