Effect of the Interaction of Kinetin with Indole Butyric Acid on the Multiplication of Steva Plant (Spanty) In Vitro

Abstract

This study was conducted in the laboratory of plant tissue culture in the College of Agriculture, University of Diyala for the period 2022-2023 to find an integrated program for propagating and multiplying Stevia outside the living body using some auxins and cytokinins and their interaction in the components of the nutrient medium. Tissue propagation experiments included a sterilization experiment, as a concentration of 15 mg.L-1 of sodium hypochlorite which gave the best sterilization percentage of 100%, and the best time period of 15 minutes gave 76.66%. Regarding the interaction between Kin and IBA, the control was superior in plant height, number of leaves, fresh weight and dry weight. Whereas, the number of branches was superior to the concentration treatment of 2.0 mg.L-1, as it gave the best number of branches, reaching 3.46 branch.plant-1. As for the effect of NAA and IBA on the rooting, NAA was given at a concentration of 0.4 mg.L-1, the best root length was 1.94 cm, and the best number of roots was 6.6 root.plant-1. As for the acclimatization experiment, there was no significant differences between the media used. This may be attributed to the genetic ability of this variety to better tolerate environmental conditions. As for the medium used in all its combinations, it proved successful in the process of acclimatizing tissue culture plants. In general, the method used did not differ from the resulting method in acclimatizing most tissue culture plants.