In Vitro Propagation of 'Petite Folie' Miniature Rose

Abstract

Cultured stem nodal sections from a greenhouse-grown miniature rose (Rosa hybrida L. 'Petite Folie') proliferated shoots after 5 weeks on Murashige and Skoog (MS) medium with the following ratios of growth regulators: 0, 0.1, 0.3, 1, 10, or 30 mg/liter 6-benzyladenine (BA) to 0, 0.1, 0.3, or 1 mg/liter napthaleneacetic acid (NAA). Use of 3 mg/liter BA alone or 3 mg/ liter BA plus 0. 1 mg/liter NAA resulted in the greatest shoot multiplication. Comparisons were made for shoot multiplication among MS, Schenk and Hildebrandt (SH), and Woody Plant Medium (WPM) media supplemented with 3 mg/liter BA and 0.05 mg/liter NAA. MS medium was most effective. No further increase in multiplication was obtained by extending the length of the culture period beyond five weeks. The microshoots were rooted in vitro after eight days on 0.5 strength MS medium supplemented with 0.5 mg/liter NAA or 0.1 mg/liter indolebutyric acid (IBA). Miniature roses are traditionally propagated by stem cuttings. However, hybrid tea, climber, and floribunda roses are usually propagated by budgrafting of the desired cultivar onto a different rootstock. In vitro clonal propagation of roses is of great commercial value. Using this technique, many pathogen-free rose plants can be produced. Tissue culture propagation of Rosa hybrida cultivars and Rosa species from isolated shoot tips has been successful (Davies, 1980; Elliott, 1970; Hasegawa, 1980; Jacobs et al., 1969, 1970a, 1970b; Khosh-Khui and Sink, 1982a, 1982b). The use of tissue culture techniques should minimize the time necessary for the introduction of new cultivars into the commercial market, and thus increase the availability of rose plants with improved horticultural characteristics. The use of in vitro culture for the propagation of cut flower and outdoor rose types requires that these roses perform at least as well on their own roots as on grafted rootstocks (Buck, 1951; Holley, 1969). This paper reports the effects of different media supplemented with selected levels of auxin and cytokinin on shoot multiplication. It also reports the effect of auxin on rooting cultured 'Petite Folie' shoots. This content downloaded from 207.46.13.11 on Wed, 10 Aug 2016 06:33:03 UTC All use subject to http://about.jstor.org/terms VOLUME 94, NUMBERS 3-4 117 Table 1. The basal media and supplements used in the in vitro propagation of 'Petite Folie' miniature rose.