In vitro propagation of Bambusa vulgaris cv. Wamin by axillary shoot proliferation

Abstract

Development of rapid in vitro cloning through axillary shoot proliferation from mature clump for the production of qualityplanting material has been described. Explants from 12-year old field grown clump of Bambusa vulgaris cv. Wamin (BVW) fromInstitute of Wood Science and Technology, Bangalore were used for initiation of cultures. Axillary bud break was achieved 95%of cultures in BVW on MS (Murashige and Skoog) liquid medium supplemented with additives and various combination of auxinsIAA, NAA(0.25mgl-1) and cytokinins viz., Kinetin, BAP (0.5-2.5mgl-1), TDZ (0.05-0.25mgl-1 ) . Additives ascorbic acid (50mgl-1),citric acid (25mgl-1) and cysteine (25mgl-1) were added during shoot initiation and shoot multiplication. The proliferated shoots (2-3 weeks old) showed rapid multiplication with 27-28 shoots/clump at fifth passage on MS liquid medium supplemented withadditives and BAP (5.0mgl-1). A clump of atleast 3 shoots were used for in vitro root induction in half- strength MS medium withNAA (1.0mgl-1). Field transfer of in vitro rooted plantlets were carried out by transferring the plantlets to pots containing sand:soil: FYM (farmyard manure) in 1:1:1 ratio, maintained in nursery at 30±2°C and relative humidity of 80±5%. Micropropagatedplants achieved a height of 30-35 cm with 3-4 tillers (shoots) with miniature rhizome within four months.