Abstract
Multiple shoots emerged from the nodal shoot segments of the field-grown candidate plus clump explants of Bambusa pallida Munro when cultured on Murashige and Skoog (MS) liquid medium with additives (ascorbic acid 50 mg/L + citric acid 25 mg/L + cysteine 25 mg/L) and combined use of α-naphthalene acetic acid (NAA) 1.34 μM + thiodiozuron 1.125 μM in a 2-week period. Further shoot multiplication was achieved in MS liquid medium with additives + NAA 1.34 μM + 6-benzylaminopurine 4.4 μM at 25 ± 2 °C and 33.78 μmol photons m−2 s−1 light illumination for a 12-h photoperiod. These shoots were rooted within four weeks in MS/2 basal salt medium with additives +2% sucrose +1% glucose, and 0.6% agar by pulse treatment of shoots with indole 3 butyric acid 0.5 mg/mL for 30 min prior to inoculation. Rooted plants were successfully hardened in the mist chamber. Survival rate during hardening was more than 95%. Micropropagated plants achieved a height of 25–30 cm with 3–4 tillers (shoots) with miniature rhizome in a 4-month period. Genetic stability was observed in the micropropagated plants.
Highlights
Arunachal Pradesh Forest Research Institue, on in vitro cloning of B. pallida through axillary Itanagar, North Eastern India, based on the shoot proliferation from the explants of superimorphological traits as well as by the Rain or genotypes of mature clumps and evaluationBambusa pallida Munro is one of the most Forest Research Institute, Jorhat.[9]
Out of 25 primers tested, 12 produced quality reproducible amplification products and these primers were selected for Random Amplified Polymorphic DNA (RAPD) amplification of the mother plant and 10 randomly selected plantlets derived from axillary shoot proliferation
DNA fingerprinting with RAPD markers was performed three times and only quality reprokept in a polytunnel at 90% relative humidity and internal DNA amplification ducible bands in the range of 350-2500 bp were scored
Summary
Arunachal Pradesh Forest Research Institue, on in vitro cloning of B. pallida through axillary Itanagar, North Eastern India, based on the shoot proliferation from the explants of superimorphological traits as well as by the Rain or genotypes of mature clumps and evaluation. Bambusa pallida Munro is one of the most Forest Research Institute, Jorhat.[9] In vitro of genetic fidelity of micropropagated plants industrially important bamboo species. It is dis- propagation of bamboo through axillary shoot through RAPD analysis. Tripura, Assam, North Bengal, edulis, D.brandisii, D.giganteus, Pseudoxyte - Meghalaya, Sikkim and Myanmar.[1] It naturally nanthera stocksii, Bambusa balcoa, Guadua Materials and Methods occurs on gentle slope at altitudes up to 2000 m angustifolia and B.glaucescens.[10,11,12,13,14,15,16,17] and in the plains It is cultivated in the planes, mostly in North-Eastern India. The (Candidate Plus Clumps) of Bambusa pallida [International Journal of Plant Biology 2012; 3:e6]
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