Abstract
We report the micropropagation of Bacopa monnieri L. a medicinal plant from Shervarayan Hills, Salem, Tamil Nadu, India. Effect of auxins (IAA, NAA & 2, 4-D) and cytokinins (BA, KIN & TDZ) on shoot induction and IAA and TDZ on root induction were assessed. Callus induction on Murashige and Skoog’s medium (MS) supplemented with NAA, 2,4-D and TDZ at various combinations/concentrations were also investigated. The auxiliary buds formed from both leaf and nodal explants on hormone free MS medium within 9 d. Growth hormones at different combinations brought out remarkable variations in shoot, root and callus induction. Rooted plantlets were transferred to 10 different soil mixtures with various ratio/combinations of biological and natural fertilizers such as humus rich soil alone (control), soil with vesicular arbuscular mycorrhizae (VAM) (10:1 & 10:2) soil with vermicompost (4:1 & 4:2) soil with farmyard manure (4:1 & 4:2) soil with flyash (1:1 & 1:2) and soil with agropeat (4:1 & 4:2) in sterile polythene bags. When the plantlets were transplanted to the actual field, this pre-adaptation increased their survival as much as 80-90% (observed for 45 days). Keywords: Bacopa monnieri L., growth hormones, auxiliary shoot proliferation, transplantation.
Highlights
WHO estimated that 80% of the population of developing countries rely on traditional medicines, mostly plant drugs, for their primary health care needs
We report the micropropagation of Bacopa monnieri L. a medicinal plant from Shervarayan Hills, Salem, Tamil Nadu, India
The auxiliary buds formed from both leaf and nodal explants on hormone free MS medium within 9 d
Summary
WHO estimated that 80% of the population of developing countries rely on traditional medicines, mostly plant drugs, for their primary health care needs. The developed nations are looking for eco-friendly treatment of various diseases through plant based source. Many valuable herbal drugs have been discovered by knowing that particular plant was used by the ancient folk healers for the treatment of some kind of ailment (Ekka & Dixit, 2007). Bacopa monnieri L. is used as a brain tonic to enhance memory development, learning and concentration and to provide relief to patients with anxiety or epileptic disorders. Memory, learning and concentration and results support the traditional ayurvedic claims. Epilepsy, bronchitis and asthma, irritable bowel syndrome and gastric ulcers supports the ayurvedic uses of Bacopa (Shakoor et al, 1994)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
