In vitro propagation of Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. from shoot tip explants

Abstract

ABSTRACTA highly efficient in vitro micropropagation protocol, using newly emerged sprouts coming from greenhouse-grown plants, was developed to produce Actinidia melanandra Franch. and Actinidia rubricaulis Dunn. This is the first report of in vitro culture of A. rubricaulis Dunn. Axenic culture was possible using one- or two-node explants or terminal buds, surface-sterilised with commercial bleach tablets. Nearly 90% success rate in establishment was observed. The best axillary shoot proliferation and maximum adventitious shoots elongation were achieved on Quoirin-Lepoivre medium supplemented with 1.5 mg L−1 6-benzyladenine and 30 g L−1 sucrose. Although used as an inert support, agar brand has a significant effect. Kobe agar at 8 g L−1 gave the best response. 100% of in vitro rooting was observed. Roots length and their quality were highly improved by the use of vermiculite in transfer medium. Over 99% of rooted plants survived after acclimatisation.