Abstract
This exercise was developed for a plant propagation course to demonstrate the 4 stages of micropropagation, the effects of cytokinin concentrations, and the differences between adventitious and axillary shoot proliferation in a short time. Greenhouse-grown stock plants were brought into the laboratory and 4-5 cm long nodal stem segments or shoot tips were surface disinfested for 15 min. in 0.5% NaClO with 1 ml/liter Tween 20 followed by 2 5-min. rinses in sterile water. Working in the open laboratory near the bases of pairs of lit Bunsen burners, students placed either single node or shoot tip explants (2 cm long, 5 reps) onto MS medium with 0, 1, or 10 μM BA in 150 × 25 mm culture tubes. Cultures were incubated on open laboratory benches. Axillary shoots grew with 0 or 1 μM BA and most callus and adventitious shoots grew with 10 μM BA. Microshoots were rooted and acclimatized under mist in the greenhouse.
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