Abstract
ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.
Highlights
RESUMO: Esta pesquisa descreve um protocolo de micropropagação eficiente para Lippia origanoides (Verbenaceae)
Lippia origanoides is an aromatic shrub native to Central America and northern region of South America (VICUÑA et al 2010). It is characterized by its antibacterial, antifungal, antiviral, antiparasitic, antioxidant, antigenotoxic, and anticancer biological activities (CELIS et al 2007; MENESES et al 2009; OLIVEIRA et al 2007; QUINTERO-RUIZ et al 2017; RAMAN et al 2017; SANTOS et al 2004; STASHENKO et al 2014), attributed to components of its essential oil and extracts
It has been shown that L. origanoides has at least five chemotypes according to its essential oil major component (A, B, C, D and E) used in the cosmetics and pharmaceutical industries (RIBEIRO et al 2014; SILVA et al 2009; STASHENKO et al 2010)
Summary
The inflorescences of L. origanoides plants (A chemotype), were collected from experimental gardens at “Centro Nacional de Investigaciones para la Agro industrialización de Especies Vegetales Aromáticas Medicinales Tropicales (CENIVAM)”, located at Universidad Industrial de Santander, (Bucaramanga, Colombia) These inflorescences were dried in the oven for 24 h at 34 °C until their use. Plantlets with well-developed roots (root length greater than 3 cm) were removed from the culture medium and washed with distilled and sterilized water to remove the adhering medium They were transplanted in sealable plastic bags with different substrates such as river sand, black peat and vermicompost, and all were previously sterilized (Figure 1). In vitro germination of L. origanoides seeds collected from the field was performed to obtain axenic seedlings as sources of explants for plant propagation.
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