In vitro proliferation of Lebanese Lemna minor and Lemna gibba on different nutrient media

H Ghanem,A Haddad,S Baydoun,H Abou Hamdan,S Korfali,L Chalak

doi:10.1080/16583655.2019.1597450

Abstract

This study aimed to assess the ability of two Lebanese duckweed species, (Lemna minor and L. gibba), to grow under in vitro conditions on three nutritive solutions, Murashige Skoog (MS), Schenk-Hildebrand (SH) and Algal Assay Procedure (AAP). Plant growth of both species, expressed as doubling time, differed significantly (p < .05) between the tested media with best results obtained on SH for L. minor after 7 days of culture and on AAP for L. gibba after 2.7 days for both species. Growth index was significantly higher (P < .05) for L. minor on SH reaching 41.6 after 21 days of culture whereas L. gibba exhibited its highest growth index of 15.51 on AAP. These results indicate the efficiency of SH and AAP media in promoting the vegetative proliferation of L. minor and L. gibba to further provide a good source of duckweed material for phytoremediation applications.

Highlights

IntroductionLemna (duckweed) is a small-sized freshwater floating macrophyte from the family Lemnaceae
Lemna is a small-sized freshwater floating macrophyte from the family Lemnaceae
Different effects of the nutrient media SH, Murashige Skoog (MS) and Assay Procedure (AAP) on the vegetative growth of L. minor and L. gibba during in vitro cultivation are demonstrated in this study

Summary

Introduction

Lemna (duckweed) is a small-sized freshwater floating macrophyte from the family Lemnaceae. In spite of the number of reported studies on pollution concerning the Lebanese fresh waterbodies [9,10,11], few studies are concerned with the phytoremediation using macrophytes of the polluted waterbodies [12]. These efforts were mostly limited to the investigation of bio-filtration in the wetland in the Upper Litani River basin to evaluate the constructed wetland performance and determine the treatment efficiency and improvement of the water quality [13]

Objectives

Methods

Discussion

Conclusion