Abstract
Anti-tetanus toxoid ( tt) antibody (Ig) levels in the supernatant of cultured, pre-immunised equine peripheral blood mononuclear cells ( pbmc) were measured by an indirect enzyme-linked immunoabsorbent assay ( elisa). Optimal anti- tt Ig production occurred at concentrations of stimulating, purified tt of between 0001 and 0·1 μg ml −1, which varied depending on the cell concentration. Optimal anti- tt Ig production was most consistently produced when the cell concentration was 5 × 10 6 ml −1. At this cell concentration maximal anti- tt Ig was induced using 0·1 μg ml −1 tt. At a cell concentration of 5 × 10 6 ml −1 and a tt concentration of 0·1 μg ml −1 anti- TT Ig was first detectable in supernatant on day 5 of stimulation. Maximal levels of anti- tt Ig were present in the supernatant by day 10. No anti- tt Ig was produced in cultures of PBMC from non-immune animals.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
