Abstract
This article describes a fast, simple, and efficient plant regeneration protocol for Ocimum basilicum L. Two sets of experiments were performed. The first set was performed on Murashige and Skoog (MS) medium lacking zinc sulfate heptahydrate (ZnSO4·7H2O) and containing varying concentrations of indole-3-acetic acid (IAA). The second set used three different concentrations (8.6, 12.9, and 17.2 mg L−1) of ZnSO4·7H2O along with varying concentrations of IAA. In the first set, without zinc sulfate, an IAA concentration of 1.0 mg L−1 (MS4) was found to be most effective, producing a mean of 12.6 roots per hypocotyl explant, while shoots were not produced. In the second set, with zinc sulfate, a combination of 12.9 mg L−1 ZnSO4·7H2O + 1.0 mg L−1 IAA (MS11) produced significantly more shoots per explant (15 shoots) than a combination of 12.9 mg L−1 ZnSO4·7H2O + 0.5 mg L−1 IAA (MS10), which produced only six shoots. Later, the plantlets were successfully acclimatized (100%) and finally transferred to the greenhouse (ex vitro). In the O. basilicum plants grown using MS11 medium, total phenolic content and rosmarinic acid content were estimated from stem, shoot tip, and old leaf tissue of in vitro plantlets, ex vitro plantlets, and flowering plants. The highest amounts of total phenolic content (131.8 mg gallic acid equivalent g−1 DW) and rosmarinic acid (13.0 mg g−1 DW) were obtained in the old leaf tissue at flowering time. This rapid regeneration protocol for O. basilicum L. represents a major improvement over the conventional protocols for plant regeneration and propagation of this species.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: In Vitro Cellular & Developmental Biology - Plant
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.