Abstract
In vitro root cultures provide an alternative means for producing secondary metabolites of pharmaceutical and industrial importance. The present investigation is an attempt to study the effect of methyl jasmonate (MeJA) as an elicitor, on the production of rosmarinic acid through in vitro root cultures of Ocimum basilicum L. The rosmarinic acid is a preformed constitutively accumulated defense compound having several biological activities including antibacterial, antiviral, and antioxidative activity. Uniform progeny of O. basilicum L. was obtained through in vitro shoot bud multiplication as reliable source of explants. In vitro root cultures were established in one fourth strength of Murashige and Skoog (MS) liquid medium supplemented with 1.0 mg l–1 ?-napthaleneacetic acid (NAA). Methyl jasmonate, was supplemented to the root cultures at different concentrations (0.01, 0.1 and 1.0 mM), and their effect on total phenol content as well as rosmarinic acid production was studied. Rosmarinic acid was quantified by high performance liquid chromatography (HPLC). Compared to the control set of cultures 1.60 fold increase in rosmarinic acid concentration (4.05 ± 0.74 % of dry wt) was observed in elicitor treated cultures.
Highlights
Ocimum basilicum L. has been used as traditional medicine for household remedy against various human ailments from antiquity
The objective of the present study is to investigate the influence of an elicitor Methyl jasmonate (MeJA), on the biomass content and intracellular accumulation of phenolic including rosmarinic acid in root cultures of O. basilicum L. and to establish an optimal culture condition for increased as well as continuous rosmarinic acid production
Accumulation of rosmarinic acid in the roots cultured in the media (1/4 Murashige and Skoog (MS) supplemented with 1.0 mg l–1 napthaleneacetic acid (NAA)) containing different concentrations (0.01, 0.1 and 1.0 mM) of methyl jasmonate (MeJA)
Summary
Ocimum basilicum L. has been used as traditional medicine for household remedy against various human ailments from antiquity. It is widely distributed and naturalized throughout tropical and subtropical parts of Asia, Africa and America [1, 2]. This plant is a rich source of phenolic compounds including rosmarinic acid. Recent interest on O. basilicum L. has resulted from its inhibitory activity against HIV-1 reverse transcriptase [3] and chemo preventive potential due to the presence of rosmarinic acid [4]. The antioxidant activity of rosmarinic acid is reported to be correlated with the scavenging action against radicals [5, 6]. The objective of the present study is to investigate the influence of an elicitor Methyl jasmonate (MeJA), on the biomass content and intracellular accumulation of phenolic including rosmarinic acid in root cultures of O. basilicum L. and to establish an optimal culture condition for increased as well as continuous rosmarinic acid production
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