Abstract
A n efficient in vitro propagation protocol for Artemi sia amygdalina D . d eveloped here resulted in the induction of s ignificantly more number of shoo ts (36 shoots per nodal explant ) on a medium containing 10 µM benzyl amino purine (BAP) and 10 µM α - naphthaleneacetic acid (NAA) than any other treatment. Growth of the regenerated shoots in phytohormone free MS medium resulted in a significant increase in biomass, shoot height and shoot multiplication. The regenerated shoots also formed roots when transferred onto full strength and half strength MS (Murashige and Skoog) medium . Healthy in vitro raised plantlets were acclimatized and maintained in standard greenhouse conditions for further growth. T his study provides a basis for germplasm conservatio n and further investigation of this rare, medicinally important plant species.
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