Abstract
The present work was conducted to develop a simple and efficient protocol for in vitro shoot regeneration and plantlet formation from unpollinated ovary culture in Allium chinense. Ovaries that were excised from flowers 2–3 days before anthesis and cultured on induction medium with 9.05μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88μM benzylaminopurine (BAP) for 2 weeks gave the best organogenic response (56.32%). For shoot regeneration, the highest percent organogenic ovaries (64.53%) were obtained on B5 induction medium containing 9.05μM 2,4-D and 8.88μM BAP. Ovaries cultured on B5 containing 10.74μM naphtaleneacetic acid (NAA) and 0.44μM BAP exhibited maximum shoot regeneration (60.32%). Shoots elongated and produced normal plantlets when cultured on B5 medium supplemented with 10.74μM NAA for 2 weeks. The plant regeneration procedure from the ovary may be an alternative for the improvement of A. chinense by genetic engineering.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
