In vitro plant regeneration from unpollinated ovaries of Allium chinense

Abstract

The present work was conducted to develop a simple and efficient protocol for in vitro shoot regeneration and plantlet formation from unpollinated ovary culture in Allium chinense. Ovaries that were excised from flowers 2–3 days before anthesis and cultured on induction medium with 9.05μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 8.88μM benzylaminopurine (BAP) for 2 weeks gave the best organogenic response (56.32%). For shoot regeneration, the highest percent organogenic ovaries (64.53%) were obtained on B5 induction medium containing 9.05μM 2,4-D and 8.88μM BAP. Ovaries cultured on B5 containing 10.74μM naphtaleneacetic acid (NAA) and 0.44μM BAP exhibited maximum shoot regeneration (60.32%). Shoots elongated and produced normal plantlets when cultured on B5 medium supplemented with 10.74μM NAA for 2 weeks. The plant regeneration procedure from the ovary may be an alternative for the improvement of A. chinense by genetic engineering.