In vitro organogenesis from leaf explants of some dwarf mutants of Arabidopsis thaliana (L.) Heynh.

Abstract

The organogenetic capacity of five dwarf mutant strains of Arabidopsis thaliana (L.) Heynh. was generally low as compared with normal line Estland on the B5 media supplemented with 9 different combinations of NAA and BA. Mutant F produced shoots in similar frequency to normal line, but produced roots less frequently. Mutant t and St1 produced shoots at one or two combinations of NAA and BA, but no rootformation was observed at any combination. Mutant le and g produced roots at one or two combinations, but no shoot-formation was observed at any combination. The media combined with NAA at 0.1-1mg/l and BA at 0.1-0.3mg/l were available for regeneration of whole plant in normal line. However, whole plant was scarcely regenerated in the tested dwarf mutants at any combination of NAA and BA.