Growth Estimation of in vitro Cultured Callus and Plant Regeneration from Leaf Disk and Petiole Callus of Musk Strawberry (Fragaria moschata Duch.) Subcultured for 18 Months.

Abstract

Callus was induced from leaves and petioles of in vitro cultred Fragaria moschata shoots. The highest callus formation rate was obtained on MS supplemented with NAA 1.0 mg·1-1 and BA 0.025 mg·1-1. These calli were transferred to eight MS media supplemented with different combinations of NAA and BA. After 18 months with periodic subcultures, callus growth was estimated by their area during a 8-week culture period. The highest growth rate was observed on medium supplemented with NAA 0.1 mg·1-1 and BA 0.25 1 mg·1-1 and the lowest on the one supplemented with NAA 0.5 mg·1-1 and BA 0.025 mg·1-1. Callus weight was measured to determine the fidelity of the callus area calculation method as a non-destructive way of estimation the grwoth rate. High correlation was observed between callus area and callus weight (r2=0.974 to 0.996). Afler 18 months in culture, calli were transferred to different regeneration-inducing media enriched with TDZ, BA, ZEA, IBA and NAA. After nearby four months on these media, the calli regenerated shoots which when transferred to a root-inducing medium, they formed normal roots.