In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

Tiago Santana Balbuena,Vanildo Silveira,Massuo Jorge Kato,Claudete Santa-Catarina,Eny Iochevet Segal Floh

doi:10.1590/s0102-33062009000100029

Abstract

Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

Highlights

The Piperaceae family comprises 14 genera and ca. 1,950 species, of which the genus Piper is the largest, with more than 600 species described worldwide (Danelutte et al 2003)
Besides being of high commercial and economical importance, Piper species are medicinally used in different ways, such as in the Indian Ayurvedic system of medicine and in the folklore medicine of Latin America and the West Indies (Parmar et al 1997)
Phytochemical studies revealed the presence of monoterpenes, sesquiterpenes, arylpropanoids, phenylpropanoids, lignans and neolignans, most of which showing biological activity against bacteria, fungus and the trypomastigote form of Trypanossoma cruzi (Moreira et al 1995; Martins et al 2000; 2003; Campos et al 2005; 2007)

Summary

Introduction

The Piperaceae family comprises 14 genera and ca. 1,950 species, of which the genus Piper is the largest, with more than 600 species described worldwide (Danelutte et al 2003). Piper species produce many biologically active compounds, including amides, flavanones, alkaloids, propenylphenols, lignans, neolignans, benzoic acids and chromenes (Parmar et al 1997; 1998; Navickiene et al 2003; Silva et al 2002; Danelutte et al 2003; Martins et al 2003; Lago et al 2004). Phytochemical studies revealed the presence of monoterpenes, sesquiterpenes, arylpropanoids, phenylpropanoids, lignans and neolignans, most of which showing biological activity against bacteria, fungus and the trypomastigote form of Trypanossoma cruzi (Moreira et al 1995; Martins et al 2000; 2003; Campos et al 2005; 2007).

Objectives

Methods

Results