Abstract
Background: Ichnocarpus frutescens has been used as Ayurvedic herbal medicine by Gond tribes in India to treat several diseases such as diabetes mellitus and Jaundice. The growing incidence of diabetes mellitus and its complications have headed to the search of novel therapeutic approaches engrossed on preventing postprandial hyperglycemia. The practice of carbohydrate breakdown enzyme inhibitors from plant resources might be a potential approach to prevent glucose absorption with the least adverse effects. Aims: The objective of the present investigation was to provide in-vitro evidence for the potential inhibitory activity of polyphenolic extract (PPE) of Ichnocarpus frutescens on amylase and glucosidase enzymes. Methods: the in the vitro inhibitory effect of polyphenolic extract (PPE) of Ichnocarpus frutescens focused on the evaluation of pancreatic amylase inhibition, rat serum amylase inhibition and rat intestinal glucosidase inhibition of polyphenolic extract (PPE) of Ichnocarpus frutescens by in vitro. The different concentrations of polyphenolic extract were subjected to amylase and glucosidase inhibitory assay and the percentage of amylase and glucosidase inhibitory activity and IC50 values were calculated. Results: Polyphenolic extract shows appreciable pancreatic amylase inhibitory activity in vitro. The extract also showed an appreciable glucosidase inhibitory effect in a concentration-dependent manner with a moderate amylase inhibitory activity. The in vitro examination of the inhibitory effect of PPE on maltase and sucrase activities revealed that PPE inhibited rat small intestine disaccharidase ( glucosidase) activity. Conclusion: Taken together, these results suggest that inhibitory effect of PPE on amylase and glucosidase activities may delay carbohydrate digestion and absorption with subsequent lowering of blood glucose level leading to prevention of postprandial hyperglycemia in diabetes and its complication.
Highlights
Hyperglycemia is a condition characterized by an abnormal excess of sugar in the blood
The aim of the study was to show the different reactivity of porcine pancreatic amylase and rat intestinal αglucosidase with different concentrations of polyphenolic extract (PPE)
The inhibitory activity of PPE against porcine pancreatic amylase was determined in vitro by CNP-G3 and starch iodine method (Fig. 1). These results show the anti-amylase activity of PPE equivalent phenolic basis, notably for the higher dose tested, the α-amylase inhibitory activity was relatively high compared to other tested doses
Summary
Hyperglycemia is a condition characterized by an abnormal excess of sugar in the blood. Chronic postprandial hyperglycemia is widely recognized as one of the earliest disease biomarkers in the prediction of subsequent microvascular and macrovascular complications that can progress to full symptomatic Type diabetes mellitus [1,2]. Aims: The objective of the present investigation was to provide in-vitro evidence for the potential inhibitory activity of polyphenolic extract (PPE) of Ichnocarpus frutescens on α-amylase and α-glucosidase enzymes. Results: Polyphenolic extract shows appreciable pancreatic αamylase inhibitory activity in vitro. The extract showed an appreciable α-glucosidase inhibitory effect in a concentration-dependent manner with a moderate α-amylase inhibitory activity. Conclusion: Taken together, these results suggest that inhibitory effect of PPE on α- amylase and α- glucosidase activities may delay carbohydrate digestion and absorption with subsequent lowering of blood glucose level leading to prevention of postprandial hyperglycemia in diabetes and its complication
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