In-vitro Inhibition of Biofilm Formation by Pseudomonas aeruginosa isolated from Chicken

Abstract

Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen which is characterized by its ability to form biofilm, on biotic and abiotic surfaces, makes it particularly resistant to host's immune defenses and current antibiotic therapies. In present study, ten strains were isolated from 100 diseased chicken with percentage of 10%. The antimicrobial susceptibility for the 10 isolates was conducted using disc diffusion method. The most of isolates were resistant to Ampicillin (90%). While, Doxycyclin and Penicillin exhibited resistance with percentage of 70¬ % for each. The isolates were genotypically studied for the presence of virulence genes (pslA, toxA and fliC). The pslA, toxA genes were detected in all (100%) of isolates, while fliC gene was detected in 50% of isolates using conventional PCR technique. The ability of isolates to form in vitro biofilm was detected by tube method followed by investigating the effect of each acetic and citric acid on biofilm formation. All of P. aeruginosa isoalets were able to form biofilm while, the biofilm inhibition percentage was evaluated to be 80% for P. aeruginosa at weak acids concentration of 2%. The current study described the role of each acetic and citric acid as an efficient antibiofilm and antibacterial agents against multidrug resistant P. aeruginosa