Abstract
The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare. Objective To explore the efficacy of a preparation containing etidronates of lanthanide and calcium ions in regenerative repair of bone defects. Material and methods The osteoblastic MC3T3-E1 and the osteoclastic RAW 264.7 cell lines were used in in vitro experiments at the first stage of the research. The agent was postoperatively injected in a bone defect of 36 rabbits on days 3 and 5 to assess the preparation’s effect on regenerative repair of small defects with diameter of 2.5 mm. Radiometric and reactive morphological characteristics of bone tissue were evaluated at the fracture site at the beginning, middle and end of experiment. Results The above preparation was found to enhance osteogenic differentiation and stimulate accumulation of intracellular calcium in MC3T3 E1 cells. However, the preparation was not shown to inhibit RANKL-induced osteoclast differentiation in vitro. Histological and computed tomography findings demonstrated statistically significant differences between control and experimental animals (p less 0.01) and indicated to the preparation’s effect of promoting regenerative repair of small bone defects. Conclusion The series was the first to show the effect of the preparation containing etidronates of lanthanide and calcium ions as stimulating osteoblast activity in vitro and promoting early regenerative repair of small bone defects.
Highlights
The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare [1]
The primarily ionic link type being typical for calcium ions and lanthanides and the proximity to Ahrens parameter (P·α = 2.6–2.8 eV·Å5/2) [7–9] are practical for lanthanide ions being injected as a complex to be incorporated in the structure of hydroxyapatite which is the basic mineral constituent of the osseous tissue
The studies emerged as a base for synthesis of a new pharmaceutical containing the above composition conventionally named as INROK, staged exploration of its effect on in vitro osteoblast and osteoclast differentiation and its capability to improve in vivo bone regeneration
Summary
The search for new more effective techniques to repair bone fractures and defects is an urgent task of healthcare [1]. Introduction of more effective agents stimulating bone regeneration is important to improve outcomes of an injury or a disease. Studies showed that lanthanides could suppress the development of cells (osteoclasts) being responsible for resorption of osseous tissue [10–13]. A combination of diphosphonates and lanthanides appeared to enhance synergic effect intensifying bone regeneration. The studies emerged as a base for synthesis of a new pharmaceutical containing the above composition conventionally named as INROK, staged exploration of its effect on in vitro osteoblast and osteoclast differentiation and its capability to improve in vivo bone regeneration
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
