Abstract
We investigated the susceptibility to Usutu virus (Flavivirus) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. Vero, PK-15, and goose embryo fibroblast cells developed cytopathic effects; however, viral multiplication was detected in all mammalian cell types by immunohistochemical tests. Chicken embryo fibroblast cells and chicken embryos were resistant.
Highlights
We investigated the susceptibility to Usutu virus (Flavivirus) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos
The appearance of cytopathic effects (CPEs) in flavivirus-infected cell cultures depends on the virus and host cell type, as well as on multiplicity of infection (MOI) levels and incubation time employed (8)
This study demonstrates that Usutu virus can infect cell cultures of various tissue types derived from a wide variety of animal species, including cell lines of human origin
Summary
We investigated the susceptibility to Usutu virus (Flavivirus) of 13 permanent cell lines, 3 primary cell cultures, and chicken embryos. The Study We investigated the in vitro susceptibility of various cell cultures and embryonated eggs to Usutu virus infection. To 1-day-old confluent monolayers of the permanent cell lines and primary cell cultures, grown on the surface of chamber slides, the Austrian Usutu virus strain Vienna 2001-blackbird
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