In vitro growth of different chrysanthemum cultivars on murashige and skoog medium modified with some organic additives

Abstract

Mass in vitro propagation of chrysanthemum plants requires large amounts of Murashige and Skoog (MS) media. In order to increase efficiency of MS media usage, the addition of organic materials and the selection of cultivars need to be studied. This study aimed to obtain the best modified MS media and cultivars for in vitro propagation of chrysanthemums. This research was conducted from July to September 2023 at tissue culture laboratory of Politeknik Negeri Jember. This study used Completely Randomized Design with two factors. The first factor was the chrysanthemum cultivars, i.e. Naweswari Agrihort and Suciono Agrihort. The second factor was the modified MS media, i.e. MS, ½MS + 50 g/l banana, ½MS + 50 ml/l coconut water and ½MS + 50 g/l sweet potato. Each combination of treatments was thrice repeated. Observation variables were plant height, time of shoot emergence, the number of shoots and the number of leaves. Data analysis was carried out using ANOVA and DMRT. The best modified media was ½MS + 50 g/l sweet potato that showed significantly higher plant compared to other treatments. The addition of the tested organic materials could reduce MS media usage by 50%.