In vitro Growth and Multiplication of Pineapple under Different Duration of Sterilization and Different Concentrations of Benzylaminopurine and Sucrose

Abstract

Objective:To analyze mechanism of multiple shoot of mutant.Method:The two-dimensional electrophoresis(2-DE) was employed to compare the global protein patterns between normal and mutant.The 2-De gel maps were evaluated qualitatively and quantitatively with the Image Master 2-DE analyzer;Results:The 59 expressed proteins in the mutant were different in quality and quantity from those in the normal,in which 35 spots increased and 20 spots decreased in quantity.Furthermore,4 spots were missing in mutant;Conclusion:The difference of maps of two stages might relate to multiple shoot of mutant.