Abstract
Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) and elicitors (chitosan, methyl jasmonate) on C. roseus hairy roots (CHR) growth has been examined in order to increase the content of vincristine and vinblastine. Our results showed that CHR generated by an Agrobacterium rhizogenes strain isolated in Vietnam was capable of producing both vincristine and vinblastine when subjected to precursors, but only vinblastine when exposed to elicitors. However, both precursors and elicitors were evaluated to have an effect on increasing the accumulation of TIAs in CHR. In particular, the use of elicitors required more time to find the appropriate induction conditions, while the use of precursors gave outstanding efficiency in the treatment with 1 µM phenylalanine. The greatest yields of vincristine (51.99 µg g-1 DW) and vinblastine (699.92 µg g-1 DW) were obtained in the 7th week (with 0.306 g DW biomass). This result is the first time we might boost the levels of vincristine and vinblastine in our CHR clone generated by the Vietnam strain of A. rhizogenes.
Highlights
Catharanthus roseus L. is a tropical perennial medicinal plant in the family Apocynaceae
Our results showed that C. roseus hairy roots (CHR) generated by an Agrobacterium rhizogenes strain isolated in Vietnam was capable of producing both vincristine and vinblastine when subjected to precursors, but only vinblastine when exposed to elicitors
Catharanthus roseus has received much pharmaceutical attention because of its chemical composition, which contains more than 130 different indole terpenoid alkaloids (TIAs), some of which are pharmacologically important and potent, such as vinblastine and vincristine
Summary
Catharanthus roseus L. is a tropical perennial medicinal plant in the family Apocynaceae. Catharanthus roseus has received much pharmaceutical attention because of its chemical composition, which contains more than 130 different indole terpenoid alkaloids (TIAs), some of which are pharmacologically important and potent, such as vinblastine and vincristine. These were demonstrated with the high effectiveness of antioxidant, hypertension, diabetes and cancer treatments [5, 6]. Hairy root is formed by a mediate interaction between soil bacterium Agrobacterium rhizogenes and plant This transformation process has delivered a precious by-product, “hairy root” that can develop unlimitedly in culture media [10]. The effects of precursors (types and concentrations) and elicitors (types, dosages, and time exposure) on CHR growth and alkaloids (vinblastine and vincristine) accumulation were evaluated
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