Abstract
AbstractRegenerated adventitious buds in an Agrobacterium‐mediated genetic transformation system of castor (Ricinus communis L.) are difficult to develop in intact plants. Therefore, an in vitro grafting system in castor was established in this study. The stocks and scions were obtained from castor seedlings, and complete castor plants could be gained by in vitro grafting. To promote growth of grafted seedlings, the effects of indole 3‐butyric acid (IBA), 6‐benzyl aminopurine (6‐BA), kinetin (KT), sodium nitrophenolate (SN), and diethyl aminoethyl hexanoate (DA‐6) on the development of grafted seedlings were explored. The experimental results indicated that when adding IBA, KT, and SN to the media at concentrations of 0.1, 0.2, and 0.8 mg L−1, respectively, the growth of castor grafted seedlings could be promoted strongly; however, when 6‐BA or DA‐6 was added to media, the growth and development of castor grafted plantlet were inhibited significantly. Moreover, the survival rate of transplantation of grafted seedlings was up to 74.5%. The method of in vitro grafting in castor could be used to obtain robust and complete castor plants quickly and might provide a solution to the problems of adventitious bud growing and rooting difficulty in the genetic transformation of castor.
Published Version
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