Abstract
SINCE the recognition of “capacitation of sperm” in the female tract1,2, fertilization in vitro of eggs has been successfully conducted by insemination of sperm recovered from the uterus for the rabbit3,4, hamster5 and mouse6. Successful fertilization of golden hamster eggs in vitro by epididymal (uncapacitated) hamster sperm5, in the presence of follicular fluid of hamster7 or cow8, or in the presence of rabbit tubal cyst fluid (our unpublished work), and that of Chinese hamster eggs in the presence of follicular fluid of golden hamster9, encouraged us to carry out the following experiment which demonstrated that mouse sperm can be capacitated in vitro to fertilize mouse eggs in the presence of bovine follicular fluid.
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