Abstract
Objective To access the effects of platelet-rich plasma (PRP) on the behaviour of human bone marrow-derived mesenchymal stem cells (hBMSCs), including proliferation and migration. Methods PRP was diluted with DMEM/F12, resulting in concentrations of 1%, 2%, and 5%. The proliferation of hBMSCs was examined by 2 methods: cell-number counting with the haemocytometer method and the colony-forming unit-fibroblast (CFU-F) assay. Cell migration was evaluated using the scratch wound healing (SWH) assay; after that, the recorded digital images were analysed by the Image-Analysis J 1.51j8 software to compare the cell-free areas between groups after 0, 24, and 48 hours. Results hBMSCs cultured in DMEM/F12 at PRP concentrations of 1%, 2%, and 5% were all able to proliferate and migrate. In the 5% PRP group, hBMSCs proliferated greatly with a significantly higher cell number than reported for all other groups on days 5, 7, and 9. CFU-Fs were observed in all groups, except for the negative control group. The SWH assay demonstrated that hBMSCs cultured in 2% and 5% PRP almost filled the artificial wound scratch and significantly migrated more than those of all other groups at both 24 h and 48 h. Conclusion This study indicated that, due to the significant enhancement of cell proliferation and migration, 5% PRP might be the optimal concentration that should be used to promote the potential of hBMSCs in wound healing.
Highlights
Periodontitis is one of the most common oral diseases that can be related to the general health and quality of life of patients
Effects of platelet-rich plasma (PRP) on human bone marrow-derived mesenchymal stem cells (hBMSCs) Morphology. hBMSCs were cultured from passage 3 to passage 4 in PRP experimental media at 37°C and 5% CO2
After 24 hours of culture, cells started to attach to the surface, and after 7–10 days, cells filled most of the cultivating areas with 80–90% confluence. hBMSCs had an elongated shape with a branched cytoplasm surrounding a round, large nucleus (Figure 1). ese morphological features were maintained after many passages. us, during cultivating and experimental periods with PRP, hBMSCs showed homogeneity and managed to sustain this through further passages
Summary
Periodontitis is one of the most common oral diseases that can be related to the general health and quality of life of patients. In the past few decades, the main objective of periodontitis treatment has changed from repairing tissue to regenerating it, thereby reversing the tissue destruction process caused by the disease [2]. Traditional treatments including scaling and root planing can hardly produce satisfactory results [3]. In these recent years, the using of growth factors has demonstrated outstanding therapy outcomes and has been seen as a promising approach for periodontal tissue regeneration [4]
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