In vitro evaluation of antioxidant and antibacterial activities of Eucheuma cottonii extract and its in vivo evaluation of the wound-healing activity in mice.

Abstract

Eucheuma Cottonii is a type of red algae obtained from Sabah with main active component, sulfated polysaccharide or k-carrageenan. The objective of this research was to evaluate the antioxidant, antibacterial and potential wound-healing properties in aqueous extraction of Ecottonii in order to meet the increasing demand for halal and natural cosmeceutical products. Aqueous extract of Ecottonii was investigated for active compounds by phytochemical screening and IR spectroscopy. Antioxidant activity was carried out using DPPH method, and the IC50 value was 1.99mg/mL. Antibacterial activity was examined against Staphylococcus Aureus using Kirby-Bauer disk diffusion method and showed 10.03±0.06mm zone of inhibition, achieved by 200mg/mL of extracts. A wound was made by skin excision of area around 100mm2 on each mouse. Test group was treated with aqueous extract gel (10% w/w); meanwhile, the mice that were treated with honey acted as the positive control group and the untreated mice as negative control group. Results showed that the wound contraction rate inclined to aqueous extracts as compared to untreated group (P<.05). Percentage of wound healing for aqueous extracts and untreated group were 87.7%±2.0% and 57.6%±5.3%, respectively. Aqueous extract was found to be comparable to the honey in wound healing.