In-Vitro Evaluation of Anti-Glycation Potential of Bruguiera Sexangula

Abstract

Diabetes mellitus (DM) is a metabolic disorder that affects people all over the world and causes hyperglycemia. Further, chronic hyperglycemia may lead to non-enzymatic protein glycation where the excess blood glucose reacts with free amino acids in proteins to form a labile Schiff's base. Then the base gets stabilized to form advanced glycation end products (AGEs), one of the hallmark compounds of glycation. Therefore, antiglycation therapy is considered as one of the important aspects of post diabetic treatment. Recently, many phytochemicals have been reported for their antiglycation potential. In the present study, Bruguiera sexangula an medicinal mangrove plant from the Eastern coastal regions of Odisha has been evaluated for its antiglycation potential using in vitro techniques. The anti-glycation activities of aqueous bark extract of this plant were determined by bovine serum albumin-fructose glycation model. The multistage glycation markers-fructosamines, thiols and β aggregation of albumin using amyloid-specific dyes–Congo red were evaluated. The studies were carried out for the 0th,1st, 2nd , 3 rd and 4th consecutive weeks. The present study showed aqueous bark extract of Bruguiera sexangula at 1mg/ml concentration could inhibit amyloid cross beta aggregation in the 0th, first, third and the fourth week. However, no inhibition was observed in the second week. On the other hand, the standard anti-oxidant compound Quercetin could inhibit the amyloid beta aggregation in all the 4 weeks in the Congo red assay. And the extract could only show amadori product inhibition potential during the 1st and the 4th week of study. While, the compound Quercetin could inhibit the amadori product in 1st, 3rd and 4th week of the fructosamine assay. Free thiols were significantly protected from oxidation by the plant extracts. The active phytocompounds were isolated by column chromatography by using different fractions of solvents (petroleum ether, chloroform and methanol) on the basis of their polarity and it is stored for further assays. The results of the present study indicated that B.sexangula can provide protection against long term glycation associated complications. However, more in depth studies need to be carried out to decipher its antiglycation potential. Further, the bioactive phytochemicals responsible for this activity may be isolated to access its antiglycation potential in suitable model organism.