Abstract
Functionalizing biomaterials with peptides or polymers that enhance recruitment of endothelial cells (ECs) can reduce blood coagulation and thrombosis. To assess endothelialization of materials in vitro, primary ECs are generally used, although the characteristics of these cells vary among the donors and change with time in culture. Recently, primary cell lines immortalized by transduction of simian vacuolating virus 40 large T antigen or human telomerase reverse transcriptase have been developed. To determine whether immortalized ECs can substitute for primary ECs in material testing, we investigated endothelialization on biocompatible polymers using three lots of primary human umbilical vein endothelial cells (HUVEC) and immortalized microvascular ECs, TIME-GFP. Attachment to and growth on polymer surfaces were comparable between cell types, but results were more consistent with TIME-GFP. Our findings indicate that TIME-GFP is more suitable for in vitro endothelialization testing of biomaterials.
Highlights
Implantation of medical devices that come into contact with circulating blood is associated with the risk of coagulation and thrombosis
To evaluate the impact of various biocompatible polymers (PMEA, poly(2-hydroxyethyl methacrylate) (PHEMA), poly(2-methoxyethyl acrylate) (PMEA)/PHEMA co-polymers, PMe3A, poly(tetrahydrofuran-2-ylmethyl vinyl ether) (PTHFVE), and poly(2-ethoxyethyl vinyl ether) (PEOEVE)) on endothelialization, human umbilical vein endothelial cells (HUVEC) were seeded on polymer-coated polycarbonate discs, and cell attachment and growth were evaluated
Morphology similar to that of cells in dish cultures, in line with our previous reports [32, 33]. These results suggest that cells attach to PMEA and PMe3A independently of integrin, but require integrin to attach to PTHFVE
Summary
Implantation of medical devices that come into contact with circulating blood is associated with the risk of coagulation and thrombosis. Material surfaces coated with bioactive molecules such as proteins from matrix, peptides, and growth factors that enhance the attachment of endothelial cells (ECs) (i.e., endothelialization) have been developed [13,14,15,16,17,18]. We used three lots of primary human umbilical vein ECs (HUVECs) and immortalized human microvascular ECs (TIME-GFP) to investigate endothelializaion on biocompatible polymers that selectively recruit ECs but exhibit antifouling activity against blood cells.
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