In vitro effectiveness of boron neutron capture therapy for glioma cells: proliferation, apoptosis and migration

Abstract

Objective To investigate the proliferation,apoptosis and migration of boron neutron capture therapy(BNCT) for C6 rat glioma cell-line.Methods The C6 cells,cultured by 10 B-DMEM and with irradiation by in-hospital neutron irradiator (IHNI),were divided into three groups:BNCT 3 Gy (Group Ⅰ),BNCT 6 Gy (Group Ⅱ),untreated control (Group lⅢ).The MTT assay was used to indentified the cell proliferation and draw the cell growth curve at 1-7 days of post-irradiation.The apoptosis rate was detected by flow cytometer (FCM) after 72 h of irradiation.Cell scratch test was performed to investigate the cell migration at 72 h of post-irradiation.Results One-well cell number of each group accounts to (7.90 ± 0.23) × 105,(5.40 ± 0.35) × 105 and (9.42 ± 0.23) × 106.Both Group Ⅰ and Ⅱ were with significant difference from Group Ⅲ(P ＜0.01).The apoptosis rates of three groups after 72 h of irradiation were (37.3 ± 0.7) ％、(84.7 ± 2.3) ％ and (23.1 ± 1.4) ％,each group showed statistic significancant difference to the others (P ＜ 0.01).Moreover,the C6 cells showed decreased migrating ability at 72 h after irradiation.Conclusions By using boron neutron capture therapy,it can effectively inhibit glioma cells' proliferation,induce apoptosis and weaken its migrating ability. Key words: Boron neutron capture therapy; C6 rat glioma cell; In-hospital neutron irradiator; In vitro