Boron neutron capture therapy induced apoptosis in SHG44 glioma cells

Abstract

Objective To investigate the inhibition of boron neutron capture therapy(BNCT) on SHG44 glioma cell line and its mechanism. Methods Methyl thiazolyl tetrazolium(MTY) assay was used to measure the level of the proliferation of SHG44. GE staining, Hoechst33342 fluorescence staining, transmission electron microscope(TEM) were applied to observe the changes in cell morphology. The effects of BNCT on cell apoptotic rate was observed by flow cytometer (FCM). The expression of Bcl-2 and Bax protein was measured by Western blot. Results The proliferation of SHG44 was obviously inhibited by BNCT in a dose-dependent manner. The results of FCM showed that the apoptotic cell rate 48 h after irradiation with 4 and 8 Gy 63.2% and 88.3%, respectively. Western blot analysis showed BNCT promoted Bax protein expression, but inhibited Bcl-2 expression. Conclusions BNCT inhibits the proliferation of SHG44 cell, induces apeptesis and promote the expression of Bax but inhibits the expression of Bcl-2. Key words: Boron neutron capture therapy(BNCT); SHG44; Apoptosis