Abstract
New bioactive materials have been developed for retrograde root filling. These materials come into contact with vital tissues and facilitate biomineralization and apical repair. The objective of this study was to evaluate the cytocompatibility and bioactivity of two bioactive cements, Bio-C Repair (Angelus, Londrina, Pr, Brazil) and TotalFill BC RRM putty (FGK, Dentaire SA, La-Chaux-de-fonds, Switzerland). The biological properties in human periodontal ligament stem cells (hPDLSCs) that were exposed to Bio-C Repair and TotalFill BC RRM putty were studied. Cell viability, migration, and cell adhesion were analyzed. Moreover, qPCR and mineralization assay were performed to evaluate the bioactivity potential of these cements. The results were statistically analyzed using ANOVA and the Tukey test (p < 0.05). It was observed that cell viability and cell migration in Bio-C Repair and TotalFill BC RRM putty were similar to the control without statistically significant differences, except at 72 h when TotalFill BC RRM putty was slightly lower (p < 0.05). Excellent cell adhesion and morphology were observed with both Bio-C Repair and TotalFill BC RRM putty. Both cements promoted the osteo- and cementogenic differentiation of hPDLSCs. These results suggest that Bio-C Repair and TotalFill BC RRM putty are biologically appropriate materials to be used as retrograde obturation material.
Highlights
In our daily practice, it is common to find in some problematic cases with lesions such as external or internal resorption, iatrogenic perforations, or periapical lesions that have a doubtful prognosis especially when there is a radiolucent area adjacent to the perforation site, and it is necessary to perform apical surgery with a retrograde root filling [1,2].Appl
This study aims to compare the cytotoxic effects and the bioactivity potential of two premixed materials used in retrograde root filling surgery such as Bio-C Repair (BCR) and TotalFill BC RRM putty (TFB) with PDLSCs
fluorescence-activated cell sorting (FACS) analysis showed a mesenchymal phenotype of cells isolated from periodontal tissues
Summary
It is common to find in some problematic cases with lesions such as external or internal resorption, iatrogenic perforations, or periapical lesions that have a doubtful prognosis especially when there is a radiolucent area adjacent to the perforation site, and it is necessary to perform apical surgery with a retrograde root filling [1,2].Appl. It is common to find in some problematic cases with lesions such as external or internal resorption, iatrogenic perforations, or periapical lesions that have a doubtful prognosis especially when there is a radiolucent area adjacent to the perforation site, and it is necessary to perform apical surgery with a retrograde root filling [1,2]. The material is placed in the root-end cavity sealing the entire area [3,4,5]. This area is where the stem cells from the periodontal ligament (PDLSCs) are located and are in direct contact with the retrograde root filling material [6,7]. It is crucial to conduct cytocompatibility studies of these calcium silicate-based materials and because it is a complicated and challenging treatment, we need the properties of the materials to be optimal [10]
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