In-vitro Drug Release and Ex-vivo Corneal Permeation of Dexamethasone and Curcumin Nanodispersions Using Facilitated Simultaneous RP-HPLC Technique

Abstract

Since the designs of optimal ocular nanodispersion formulations for dexamethasone and curcumin permeation via the cornea are lacking, the aim of this study was to establish the corneal permeability of dexamethasone and curcumin across goat eyes. For that, a facilitated RP-HPLC method with PDA detection has been developed and validated for the simultaneous estimation of dexamethasone and curcumin. The chromatographic estimation was achieved using Eclipse XDB C8 analytical column and the mobile phase consisting of acetonitrile: water (42: 58 % v/v) run at a flow rate of 1.0 mL/min with an injection sample volume of 200 μL. The UV detection was carried out at 281 nm using PDA detector. The developed method was able to separate the drug peaks with retention time of 3.5 and 10.8 min for the dexamethasone and curcumin, respectively. The method was validated for its linearity, system precision, method precision, analyst variation, inter/ intraday variations, detection limits and robustness. Hence the developed method was found to be selective and sensitive as both peaks were well separated with a moderate analysis time and could be used for the routine analysis in drug developmental research.