Abstract
The partial purified resveratrol was obtained from the skin of black grape fruit cultivated in Iraq using 80% ethanolic solution, then an acid hydrolysis with 10% HCl solution for (10–30) min at 60Cº was carried out. The aglycone moiety was taken with an organic solvent (chloroform), then using an open glass column packed with silica gelG 60 as a stationary phase and a mobile phase of; benzene: methanol: actic acid (20:4:1). The study utilized an in vitro evaluation for the cytotoxic effect of the partially purified resveratrol on some cell lines including, the murine mammary adenocarcinoma (Ahmed –Mohammed –Nahi–2003 -AMN -3) cell line; the human laryngeal carcinoma (Hep -2) cell line and the Rat Embryo Fibroblast (REF) cell line at different concentrations and different exposure time of treatment. The partial purified resveratrol extract concentrations ranging (7.8–4000) µg/ml in a two fold serial dilutions were used to treat the three types of cell lines for 48 and 72 hours intervals. AMN-3 cell lines showed highest sensitivity toward the cytotoxic effect of the paritial purified resveratrol than other cell lines after 48 hours in a dose dependent manner. While Hep-2 cell line showed novel behavior, the lowest concentration of cell treatment gave the most significant (P< 0.01) inhibitory effect. Only the highest concentration gave significant inhibitory effect (P< 0.01) with the transformed Ref cell line.
Highlights
The search for novel and effective cancer chemopreventive agents has led to the identification of various naturally occurring phytocompounds, one of which is resveratrol. It is a phytoalexin isolated from grape skin and other fruits [1], and black grape cultivated in Iraq is rich in resveratrol [2]
Cytotoxic Effect of Partial Purified Resveratrol on Cell Line: In vitro Study The in vitro method was used to investigate the effect of partially purified resveratrol on two types of tumor cell lines (Hep-2 and AMN-3 tumor cell lines ) and to compare the results with resveratrol effect on normal cell line Ref-2 in different concentrations and at different exposure times
Fig(2): Inhibition rate (%IR) histogram for different concentrations of partial purified resveratrol on AMN-3 cell line b. cytotoxic effect on Hep 2 cell line : The study was done on Hep 2 cell line: The effect of the partial purified resveratrol on Hep-2 cell line was shown in Figure (3)
Summary
The search for novel and effective cancer chemopreventive agents has led to the identification of various naturally occurring phytocompounds, one of which is resveratrol (trans- 3, 4 ,5 trihydroxy stilbene). 2. In vitro cytotoxic study of the partial purified resveratrol on different types of cell lines at different concentrations and exposure times . Cytotoxic Effect of Partial Purified Resveratrol on Cell Line: In vitro Study The in vitro method was used to investigate the effect of partially purified resveratrol on two types of tumor cell lines (Hep-2 and AMN-3 tumor cell lines ) and to compare the results with resveratrol effect on normal cell line Ref-2 in different concentrations and at different exposure times .
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