IN VITRO CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SOME PLANT EXTRACTS ON DIFFERENT HUMAN CANCER CELL LINES

Abstract

s. PHYTOPHARM 2012 M 50 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 concentration of OPCs and antiradical activity in tests “in vitro”. More than 40 samples of food and medicinal plants were investigated. Total polyphenolics were determined employing folin-Ciocalteu photometric method, sum of OPC — by bate-Smith acid butanol assay, free radical scavengic activity (fRSA) — by the dPPh test. The study was carried out to search the antioxidant ingredients for diet enrichment. No direct correlation between the antioxidant in vitro tests and the content of polyphenolic compounds and proanthocyanidins has been found. On the basis of the above test results the feasibility of using products based on cinnamon, red beans, cocoa and blueberry for diet enrichment has been shown. IN VITRO CYTOTOXIC AND ANTIOXIDANT ACTIVITIES OF SOME PLANT EXTRACTS ON DIFFERENT HUMAN CANCER CELL LINES © El-Hallouty Salwa M. , Khalifa Noha S. , Barakat H. M. , Salim Dina 2 Pharmacognosy Department, National Research Centre, Giza, Cairo, Egypt Botany Department, Ain Shams University, Cairo, Egypt This study involves a bioassay screening of 22 methanol extracts from 19 plants that are commonly used in Egypt for many purposes based on their ethnic values. The aim of this study is to evaluate the cytotoxic and antioxidant activities of 22 methanol extracts. The methanol plant extracts were tested in vitro against four human cancer cell lines (by using MTT method) to determine their cytotoxic effect. The cell viability was examined after 24 h exposure to 100 μg/ml of the extract in the medium. Negative (dimethyl sulfoxide) and positive (Annonacherimolia methanol extract) controls were simultaneously used. Moreover, the antioxidant effect was determined using dPPh assay. Extracts showing cytotoxic and antioxidant activities were further subjected to determine their (lethal concentration) lC 50 and lC 90 values. Confirmed in vitro cytotoxic activity was found in four plant extracts. in hepG-2, Cympopogonproximus, Perseaamericana fruits and Vignaunguiculataseeds showed lC 50 = 57.4, 13.3 and 56.4, respectively. in A-549, Perseaamericana fruits and Tabernamontanadivaricata leaves showed lC 50 = 35.4 and 70.7, respectively. in hT-29, Cympopogonproximus, Perseaamericana fruits and Tabernamontanadivaricata leaves showed lC 50 = 58.6, 22 and 67.5, respectively. in MCf-7, Perseaamericana fruits showed lC 50 = 54.5. In vitro antioxidant activity was confirmed in three plant extracts Ceratoniasiliqua leaves, Perseaamericana leaves, Abrusprecatorius seeds showed lC 50 = 10.6, 46.5 and 25.7, respectively. 5 Extracts out of the 22 studied methanol extracts exhibited potent antioxidant properties when tested at the concentration of 100 ppm against dPPh. The methanol extract of Persea americana leaves, Abrus precatorius seeds, Ceratonia siliqua leaves had lC50 = 46.5, 25.7, 10.6 μg/ml, respectively Table 4. The methanol extracts of Ocimum basillicum and hilianthus annuus had lost their activity below 100 ppm. The plants under study may represent promising natural sources for cytotoxic and antioxidant drug discovery. PHYTOCHEMICAL SCREENING AND ANTIOXIDANT CAPACITIES OF SOME COMPOSITAE PLANTS © Ergene B. , Bahadir Acikara O. , Bakar F. , Saltan G., Nebioglu S. 2 Ankara University Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey Ankara University Faculty of Pharmacy, Department of Biochemistry, Ankara, Turkey Asteraceae is one of the families which include many members containing phenolic compounds (1). The beneficial effects of many of the phenolics in human health have been attributed to their reactive oxygen and nitrogen scavenging and antioxidant capacity. The consumption of vegetables, fruits and flavonoid-rich beverages has been reported to prevent against neurodegenerative diseases, cancer and aging (2). Phenolic compounds have antioxidant potential due to their tendency to act as reducing agents, hydrogen donors, singlet oxygen quenchers and metal chelators, chelating agents which can bind metal ions, could be added to enhance the activity of natural preservatives in food stuffs (2, 3, 4). in this study, the antioxidant activity of three members of Asteraceae family; Crepis foetida subsp. rhoeadifolia, Leontodon crispus var. asper and Pilosella hoppeana subsp. testimonialis were evaluated using 1,1-diphenyl-2-picrylhydrazyl (dPPh) free radical scavenging assay and measuring malondialdehyde measuring levels. in dPPh free radical scavenging activity as-