Abstract
The cytoskeleton is a complex network of filaments whose 3D structure contributes significantly to overall function. Microtubule cytoskelton in particular has been difficult to model faithfully in vitro in 3D because microtubule networks are often sparse and structured and so cannot be modeled as a random hydrogel. We have developed a flexible and precise microtubule-based assay which allows multiple microtubules to be independently held and positioned in 3D using holographically defined optical traps. Forces exerted on microtubules in the network can be accurately quantified. We will present relevant details of the approach and its applications for molecular motor transport modeling and biomechanics experiments.
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