Abstract
The market of flowers and ornamental plants is dependent on the diversification of species and the availability of high quality propagation materials. Actually, in vitro culture techniques performance a prominent role in the multiplication and maintenance of commercially propagated ornamental plant species, and are promising for the production of thousands of high quality plants in relatively short term. In addition, when market demand for a particular species is low or zero in a specific period of the year, in vitro culture techniques allow the conservation of cultures under aseptic conditions, by Slow Growth Storage (SGS), from a few weeks to one year (or more), without affecting their viability and potential regrowth. This can be achieved by modifying the constitution of the culture medium and the maintenance conditions of in vitro cultures. Obviously, the success of the technique depends on greatly on the physiological characteristics of the species to be conserved, as well. Once a SGS protocol is optimized, the expenses labor, the possibility of contamination and the probability of somaclonal variation can be reduced markedly.
Highlights
The ornamental plant industry is expanding every year around the world (BOTELHO et al, 2015)
This is due to the availability of high quality regenerative and phytosanitary propagation materials, allowing the diversification of ornamental plants used in the various segments of the business chain, as garden plants, cut flowers and foliage, dried flowers, edible flowers, and others
The use of biotechnological tools, such as in vitro culture techniques, is, undoubtedly, one of the key components that allowed the increase of this diversification of quality materials available to the actual market of ornamental plants
Summary
The ornamental plant industry is expanding every year around the world (BOTELHO et al, 2015). Plant metabolism may be reduced by (i) reduction of temperature and / or light intensity, (ii) addition of osmotic compounds such as mannitol or sucrose, reducing the availability of water and (iii) addition of growth retardants in storage medium, especially inhibitors of gibberellin biosynthesis (GROUT, 1995; RADEMACHER, 2000; CASTRO and HILHORST, 2004; SILVA and SCHERWINSKI-PEREIRA, 2011). These approaches can be applied alone, or in combination. This review, will deepen into the technique of Slow Growth Storage for the medium-term conservation of ornamental plants
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