Abstract
The aim of the present investigation was to standardize the in vitro shoot tip culture technique considering various culture aspects for direct plant formation of two onion (Allium cepa L.) varieties ‘Indian’ and ‘Taherpuri’. About 1.5-2.2 mm shoot tips were collected from bulbs and were sterilized with 0.1% HgCl2 in various duration. Then they were cultured on MS medium supplemented with various hormonal concentrations and different media composition for primary establishment. BAP 1.5 mg l-1 + NAA1.0 mg l-1 was found to be the best formulation for primary establishment of shoot tips. Primarily established shoot tips were further cultured for shoot multiplication, and 2ip 2.0 mg l-1 + NAA 0.5 mg l-1 was found the most effective for shoot multiplication. The highest percentage of shootlet produced root on MS+2.0 mg l-1 IBA for both the cultivars (Taherpuri 90% and Indian 80%). The plantlets were transferred to small pots containing soil : sand 1:1 and kept under shade and covered with perforated polythene sheet. After proper hardening the plantlets were transferred to field.
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