In vitro clonal propagation of nicotiana tabacum with modified medium and studying its gene alteration through bioinformatic analysis

Abstract

Tobacco has medicinal uses and was used as an insecticide. Also, tobacco holds social and custom noteworthiness in different social orders so hereditary modifications and change ponders can be effectively analyzed within the test plant due to its simple genome courses of action. In this study, we treated tobacco seeds with EMS chemical and analyzed the changes in the DNA mutation compared with the control. Total nine gene sequences were taken such as AP2, CAD, CHI, PIP1, LTP1, QPT, RBOH, UPL5, PR1 to study the DNA alternations, through sequencing and comparison with mutated genes. Gene mutation was determined using the bioinformatics tool namely Bioedit. From the result obtained in bioedit were higher mutation rate was observed in PR1 followed by AP2 and CHI gene. All gene sequences were mutated except RBOH and UPL5. This preliminary study showed the effects of EMS on treating tobacco plants. This genetic alternation study can be used in constructing highly efficient tobacco lines against future TILLING studies.