In-vitro cell interaction and apatite forming ability in simulated body fluid of ICIE16 and 13-93 bioactive glass coatings deposited by an emerging suspension high velocity oxy fuel (SHVOF) thermal spray

S Bano,A Rincon Romero,D.M Grant,A Nommeots-Nomm,C Scotchford,I Ahmed,T Hussain

doi:10.1016/j.surfcoat.2020.126764

S Bano, A Rincon Romero + Show 5 more

Open Access

https://doi.org/10.1016/j.surfcoat.2020.126764

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Abstract

ICIE16 and 13-93 bioactive glasses have been proposed as alternative chemically stable compositions in physiological fluid keeping bioactivity comparable to Bioglass®. ICIE16 and 13-93 bioactive glasses coatings were produced via an emerging suspension high-velocity oxy-fuel (SHVOF) thermal spraying technique. Suspensions of ICIE16 and 13-93 with 10 wt% solid loading in isopropanol (IPA) and water were used to produce coatings on AISI304 stainless steel using a flame power of 50 kW and 75 kW. For both glass formulations, the coatings deposited at a lower flame power were more porous, less hard, and less rough (~6% porous and 242 HV) than the coatings obtained at a higher flame power (~4% porosity and 300 HV). ICIE16 coatings showed more dissolution in SBF (simulated body fluid) than the 13-93 coatings. Moreover, the 13-93 glass coating sprayed at 75 kW showed the highest stability in SBF since only 2% of the coating was resorbed in SBF after 7 days of immersion and revealed apatite precipitation after 7 days. In-vitro cell tests, using MG63 cells, showed good cell attachment and proliferation on the surfaces of the coatings, revealing good cytocompatibility. The 13-93 coating sprayed at 75 kW revealed the highest cell proliferation after 7 days of incubation. This can be attributed to the higher surface roughness of the coating (Ra = 6.5 ± 0.6 μm).

Highlights

Several bioactive silicate-based glass formulations with excellent bone bonding properties in biomedical applications have been devel oped over the years [1]
ICIE16 and 13-93 bioactive glasses coatings obtained after spraying at a flame power of 50 kW had similar micro structure; comparing the surface roughness, the 50 kW ICIE16 coating surfaces were smoother than the 13-93 bioactive glass coating deposited at the same flame power
Bioactive coatings of ICIE16 and 13-93 compositions with varied apatite forming ability were successfully produced by an emerging suspension high-velocity oxy-fuel (SHVOF) thermal spraying technique

Summary

Introduction

Several bioactive silicate-based glass formulations with excellent bone bonding properties in biomedical applications have been devel oped over the years [1]. Hench et al [4] developed the first bioactive glass, Bioglass® (45S5), containing 45% SiO2, 24.5% Na2O, 24.5% CaO and 6% P2O5 (wt%), which forms a good bond with the bone at the interface between the implant and the host tissue and is suitable for rapid bone regeneration. Low NC means high dissolution rate and apatite forming ability in physiological fluid [5]. Due to high apatite forming ability and dissolution of Bioglass®, the coatings deposited with this glass may degrade over time and subsequently resulting in instability of the implant in the long term [6], there is a need to explore coatings with new bioactive glass compositions that could survive for longer [7]

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